Antigen-Specific Proliferative Response of Peritoneal Exudate Lymphocytes Primed with Antigen and Bacterial Lipopolysaccharide

• Nitta Toshimasa

  Department of Bacteriology, Ohu University School of Dentistry

• Imai Hiroaki

  Department of Bacteriology, Ohu University School of Dentistry

• Ogasawara Yuko

  Department of Bacteriology, Ohu University School of Dentistry

• Hirayama Nobukuni

  Department of Bacteriology, Ohu University School of Dentistry

• Nakano Masayasu

  Department of Microbiology, Jichi Medical School

抄録

In vitro antigen-specific proliferation was investigated in a lymphocyte population that had been taken from the peritoneal exudate cells (PEC) of C3H/HeN mice (Ia^k) primed in vivo with both bacterial lipopolysaccharide (LPS) and horse red blood cells (HRBC) and had been purified by passage through a nylon fiber column (Nfc). The proliferative response of the Nfc-passed lymphocytes primed with HRBC and LPS [T(HRBC+LPS) cells] depended on the dose of antigen in the cultures, and the response was higher than that of cells prepared from mice primed with HRBC alone [T(HRBC) cells]. No response was seen in the cells prepared from the LPS-primed mice [T(LPS) cells] or normal mice [T(N) cells]. The response of the T(HRBC) cells was abolished by previous treatment of the cells with anti-Ia^k antibody and complement (C), whereas the response of the T(HRBC+LPS) cells was retained after the same treatment, indicating that the Ia^- T(HRBC+LPS) cells can proliferate in response to antigen in spite of Ia⁺ accessory cell-depletion. Supernatants from the cultures of Ia^- T(HRBC+LPS) cells in the presence of HRBC showed abundant IL-2 activity, while those of Ia^- T(HRBC) cells did not. The IL-2 should be produced by the L3T4 cell population in T(HRBC+LPS) cells in response to antigen, since the previous treatment of the cells with anti-L3T4 antibody and C abrogated the production. On the other hand, the Ia^- T(HRBC+LPS) cells as well as the Ia^- T(LPS) cells could respond to IL-2 dose-dependently when recombinant IL-2 was added into the cultures, but the response of Ia^- T(HRBC) cells to IL-2 was very weak. The cell population responding to IL-2 in the T(HRBC+LPS) cells as well as T(LPS) cells must be AsGM1-positive or natural killer (NK) cells, since previous treatment of the cells with anti-AsGM1 antibody and C abrogates the response. Together these results suggest that L3T4 lymphocytes capable of producing IL-2 in response to HRBC antigen without Ia⁺ accessory cells are generated in the PEC of the mice after priming with LPS and antigen together, and the IL-2 produced by the L3T4 lymphocytes induces the proliferation of the LPS-primed AsGM1⁺ cells.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 37 (12), 953-960, 1993

  Center For Academic Publications Japan

詳細情報

• CRID: 1571417128058476288
• NII論文ID: 130003483960
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00080127321?from=CiNii
• 本文言語コード: en
• データソース種別:
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