<i>In Vitro</i> Cultivation of <i>Borrelia duttonii</i> on Cultures of SflEp Cells

• Konishi Hisanori

  Department of Microbiology, Yamaguchi University School of Medicine

• Morshed Muhammad Golam

  Department of Microbiology, Yamaguchi University School of Medicine

• Akitomi Hisaji

  Department of Microbiology, Yamaguchi University School of Medicine

• Nakazawa Teruko

  Department of Microbiology, Yamaguchi University School of Medicine

Abstract

Borrelia duttonii strain 406K, a causative agent of relapsing fever, could not be cultivated in vitro in currently available media for borreliae. We have developed an in vitro cultivation system by using SflEp cell cultures. The average increases of the number of borreliae, when inoculated with 1.0×10⁵ organisms per ml from infected mice, were 23-fold and 150-fold in the primary culture and the 3rd subculture, respectively. Even a single borrelia could propagate in this cultivation system. This system will be useful for immunological and physiological studies on uncultivable Borrelia strains.

Journal

• Japanese Journal of Microbiology

  Japanese Journal of Microbiology 37 (3), 229-232, 1993

  Center For Academic Publications Japan

Details

• CRID: 1573668927872180096
• NII Article ID: 130003483968
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00079460651?from=CiNii
• Text Lang: en
• Data Source:
  • CiNii Articles