Mechanism of Inhibition of DNA Synthesis by 1-β-D-Arabinofuranosyl-<i>E</i>-5-(2-Bromovinyl)uracil

• Suzutani Tatsuo

  Division of Virology, National Institute for Medical Research

• Machida Haruhiko

  Research Laboratories, Yamasa Corporation

• Honess Robert W.

  Division of Virology, National Institute for Medical Research

抄録

The mechanism of the inhibitory action of 1-β-D-arabinofuranosyl-E-5-(2-bromovinyl) uracil triphosphate (BV-araUTP) on DNA synthesis by Escherichia coli DNA polymerase I Klenow fragment was studied. Acting as a chain terminator, BV-araUTP inhibited DNA synthesis by Klenow fragment more effectively than 2', 3'-dideoxythymidine triphosphate (ddTTP). However, the incorporation sites of BV-araU monophosphate were restricted at consecutive dTMP sequence whereas ddTMP was incorporated at every dTMP site.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 37 (6), 511-513, 1993

  Center For Academic Publications Japan

詳細情報

• CRID: 1573950402848882688
• NII論文ID: 130003483983
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00079666084?from=CiNii
• 本文言語コード: en
• データソース種別:
  • CiNii Articles