Lipofection of Synthetic Oligodeoxyribonucleotide Having a Palindromic Sequence of AACGTT to Murine Splenocytes Enhances Interferon Production and Natural Killer Activity

• Yamamoto Toshiko

  Department of Bacterial and Blood Products, National Institute of Health

• Yamamoto Saburo

  Department of Bacterial and Blood Products, National Institute of Health

• Kataoka Tetsuro

  Department of Bacterial and Blood Products, National Institute of Health

• Tokunaga Tohru

  National Institute of Health

抄録

A synthetic 22-mer oligodeoxyribonucleotide having an AACGTT palindrome, AAC-22, induced interferon (IFN) production and augmented the natural killer (NK) activity in murine splenocytes, whereas its analogue, ACC-22, having an ACCGGT palindrome, did not. The binding of AAC-22 to splenocytes was not different from that of ACC-22. Lipofection of AAC-22 to splenocytes remarkably enhanced IFN production and NK cell activity, whereas that of ACC-22 caused little enhancement. These results strongly suggest that the prerequisite for IFN production is not the binding of AAC-22 to the cell surface receptors, but its penetration into the spleen cells.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 38 (10), 831-836, 1994

  Center For Academic Publications Japan

詳細情報

• CRID: 1571417128058760064
• NII論文ID: 130003484026
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00080847632?from=CiNii
• 本文言語コード: en
• データソース種別:
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