Molecular Cloning of the Wild-Type and Mutant <i>thyA</i> Gene from <i>Shigella flexneri</i> Y

• Nur-E-Kamal Mohammed Shah Alam

  Lüdwig Institute for Cancer Research

• Mamun Abu Amar Md Al

  Bacterial Genetics Laboratory, International Centre for Diarrhoeal Disease Research

• Ahmed Zia Uddin

  Bacterial Genetics Laboratory, International Centre for Diarrhoeal Disease Research

抄録

The thyA gene which codes for thymidylate synthase has been cloned and sequenced from the wild-type Shigella flexneri Y strain SH4 and a thyA mutant TSF21 after amplifying the gene by polymerase chain reaction (PCR). The nucleotide sequence revealed 98% homology to the E. coli K-12 thyA gene. The sequence of the wild-type thyA gene of Shigella flexneri Y was identical with that of the thyA mutant except that the residue T at position 345 was replaced by residue A in the thyA mutant. This change would cause a predicted amino acid substitution of leucine at position 44 in the polypeptide product of the wild type by glutamine in the mutant. Thus, Leu⁴⁴ may be critical in enzymatic activity of the thyA gene product thymidylate synthase.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 38 (4), 309-312, 1994

  Center For Academic Publications Japan

詳細情報

• CRID: 1573105977919030400
• NII論文ID: 130003484087
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00080448242?from=CiNii
• 本文言語コード: en
• データソース種別:
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