A New Primer Pair for Detection of <i>Chiamydia pneumoniae</i> by Polymerase Chain Reaction

• Kubota Yoshifumi

  Division of Respiratory Diseases, Department of Medicine, Kawasaki Medical School

抄録

In order to improve the detection and identification of Chlamydia pneumoniae, new primers for polymerase chain reaction (PCR) were designed based on the DNA base sequence within the 53-kDa protein gene, which is specific for C. pneumoniae. The primers permitted the identification of 24 C. pneumoniae strains collected from different geographical locations, but no reaction was observed with C. trachomatis, C. psittaci nor C. pecorum. The primers were unable to amplify the DNA of bacteria commonly related to respiratory tract infections. The positive amplification was achieved with only 9 EBs/assay. Therefore, the new primers seem to be useful in the diagnosis of C. pneumoniae infections.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 40 (1), 27-32, 1996

  Center For Academic Publications Japan

詳細情報

• CRID: 1570572703128264192
• NII論文ID: 130003484288
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00082269678?from=CiNii
• 本文言語コード: en
• データソース種別:
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