Typing of Verotoxins by DNA Colony Hybridization with Poly- and Oligonucleotide Probes, a Bead-Enzyme-Linked Immunosorbent Assay, and Polymerase Chain Reaction

• Yamasaki Shinji

  Department of Microbiology, Faculty of Medicine, Kyoto University Research Institute, International Medical Center of Japan

• Lin Zaw

  Department of Microbiology, Faculty of Medicine, Kyoto University

• Shirai Hiromasa

  Department of Microbiology, Faculty of Medicine, Kyoto University

• Terai Akito

  Department of Microbiology, Faculty of Medicine, Kyoto University

• Oku Yuichi

  Institute for Diagnostic Reagents, Nissui Pharmaceutical Co.

• Ito Hideaki

  Department of Microbiology, Faculty of Medicine, Kyoto University

• Ohmura Mari

  Department of Microbiology, Faculty of Medicine, Kyoto University Research Institute, International Medical Center of Japan

• Karasawa Tadahiro

  Department of Microbiology, Faculty of Medicine, Kyoto University

• Tsukamoto Teizo

  Osaka Prefectural Institute of Public Health

• Kurazono Hisao

  Department of Microbiology, Faculty of Medicine, Kyoto University Research Institute, International Medical Center of Japan

• Takeda Yoshifumi

  Department of Microbiology, Faculty of Medicine, Kyoto University Research Institute, International Medical Center of Japan

Bibliographic Information

Other Title

• Typing of Verotoxins by DNA Colony Hybridization with Poly‐ and Oligonucleotide Probes, a Bead‐Enzyme‐Linked Immunosorbent Assay, and Polymerase Chain Reaction

Abstract

To identify the type of Verotoxins (VT) produced by Verocytotoxin-producing Escherichia coli (VTEC), a sensitive bead-enzyme-linked immunosorbent assay and polymerase chain reaction with common and specific primers to various VTs (VT1, VT2, VT2vha, VT2vhb, and VT2vp1) were developed. Together with colony hybridization tests with oligo- and polynucleotide probes, these methods were applied to VTEC isolates to type the VT produced. The toxin types of 26 of 37 strains were identified, but the reaction profiles in assays of the remaining 11 strains suggested the existence of new VT2 variants. The application of these identification procedures may be useful as a tool for clinical and epidemiological studies of VTEC infection.

Journal

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 40 (5), 345-352, 1996

  Center For Academic Publications Japan

Keywords

• Virology
• Immunology
• Microbiology

Details

• CRID: 1390282681295294848
• NII Article ID: 130003484372
• DOI: 10.1111/j.1348-0421.1996.tb01078.x
• COI: 1:CAS:528:DyaK28XjslGktr0%3D
• ISSN: 13480421
• ISSN: 03855600
• ISSN: http://id.crossref.org/issn/03855600
• PubMed: 8999287
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1348-0421.1996.tb01078.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1348-0421.1996.tb01078.x
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed