<i>In Vitro</i> Amphotericin B Effects on Growth, Viability and Dimorphism of <i>Paracoccidioides brasiliensis</i>
-
- Jesuino Rosália Santos Amorim
- Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás
-
- Soares Renata de Bastos Ascenço
- Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás
-
- Salem-Izacc Silvia Maria
- Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás
-
- Pereira Maristela
- Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás
-
- Felipe Maria Sueli Soares
- Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade de Brasília
-
- Soares Célia Maria de Almeida
- Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás
Abstract
The in vitro effects of amphotericin B deoxycholate suspension (fungizone) on Paracoccidioides brasiliensis growth, cell viability and transformation were investigated. We also analyzed the protein synthesis patterns of both cellular forms, yeast and mycelium in the presence of AmB. This drug, at 30μg/ml, highly inhibited yeast growth, which could be recovered depending on treatment time, where the most effective reversion was observed after 6hr of incubation. The yeast cell viability, that had been partially affected by the drug, could also be efficiently recovered after AmB was removed. The effect of AmB on the cellular dimorphism process showed a strong reduction in the mycelium to yeast transformation (80% inhibition compared to the control without the drug). On the other hand, the transformation from yeast to mycelium in the presence of AmB was 50% affected, relative to the control. In contrast to the growth and cell viability experiments, the reversion effects on dimorphism were partial when the drug was removed, even with only 6hr treatment. The two-dimensional gels of 35S-labeled proteins revealed a strong reduction in the three species of 80, 71 and 56kDa in yeast and mycelium when treated with AmB.
Journal
-
- Japanese Journal of Microbiology
-
Japanese Journal of Microbiology 41 (2), 93-99, 1997
Center For Academic Publications Japan
- Tweet
Details 詳細情報について
-
- CRID
- 1571698603035165568
-
- NII Article ID
- 130003484476
-
- ISSN
- 03855600
-
- Text Lang
- en
-
- Data Source
-
- CiNii Articles