Heat Shock Cognate Protein 71-Associated Peptides Function as an Epitope for <I>Toxoplasma gondii</I>-Specific CD4<SUP>+</SUP> CTL

J-STAGE
  • Yang Tian-Hui
    Department of Parasitology, Chiba University School of Medicine
  • Aosai Fumie
    Department of Parasitology, Chiba University School of Medicine
  • Norose Kazumi
    Department of Parasitology, Chiba University School of Medicine
  • Mun Hye-Seong
    Department of Parasitology, Chiba University School of Medicine
  • Yano Akihiko
    Department of Parasitology, Chiba University School of Medicine

抄録

HLA-DR-restricted CD4+ cytotoxic T-lymphocyte (CTL) lines specific for Toxoplasma gondii (T. gondii) -infected melanoma cells have been established from peripheral blood lymphocytes (PBLs) of a patient with chronic toxoplasmosis. The role of heat shock cognate protein (HSC) 71 in antigen (Ag) processing and presentation of T. gondii-infected melanoma cells to these CD4+ CTL lines was investigated. A human melanoma cell line (P36) pulsed with T. gondii-infected P36 cell-derived HSC71 was lysed by a T. gondii-specific CD4+ CTL line (Tx-HSC-1). The Tx-HSC-1 also killed T. gondii-infected P36 cells. The lytic activity of Tx-HSC-1 against P36 cells pulsed with T. gondii-infected P36 cell-derived HSC71 was inhibited by monoclonal antibodies (mAbs) against HSC71. Anti-human leukocyte antigen (HLA) -DR mAb also partially blocked the lytic activity, whereas anti-HLA-A, B, C mAb did not block the lytic activity. In addition, a flow cytometric analysis with these specific mAbs against HSC71 showed HSC71 to be expressed on the cell surface of T. gondii-infected P36 cells as well as uninfected P36 cells. These data indicate that HSC71 molecules are expressed on human melanoma cell line P36, and that HSC71 may play a potential role in Ag presentation and processing of T. gondii-infected P36 cells to CD4+ CTL.

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