Protective Effects of a Culture Supernatant of <i>Lactobacillus acidophilus</i> and Antioxidants on lleal Ulcer Formation in Rats Treated with a Nonsteroidal Antiinflammatory Drug

J-STAGE
  • Bing Shan Ruo
    Department of Bacteriology, School of Medicine, The University of Tokushima
  • Kinouchi Takemi
    Department of Bacteriology, School of Medicine, The University of Tokushima
  • Kataoka Keiko
    Department of Bacteriology, School of Medicine, The University of Tokushima
  • Kuwahara Tomomi
    Department of Bacteriology, School of Medicine, The University of Tokushima
  • Ohnishi Yoshinari
    Department of Bacteriology, School of Medicine, The University of Tokushima

抄録

Ileal ulcers and thiobarbituric acid (TBA)-reactive substances in the ileal mucosa were induced in rats treated with a nonsteroidal antiinflammatory drug, 5-bromo-2-(4-fluorophenyl)-3-(4-methylsul-fonylphenyl)thiophene (BFMeT), at a dose of 1, 000mg/kg administered with tap water as drinking water. However, the formation of ileal ulcers and TBA-reactive substances in the ileal mucosa was repressed by giving the animals a culture supernatant of Lactobacillus acidophilus as drinking water. We measured the antioxidative activity of the culture supernatant and found that the supernatant inhibited the formation of t-butyl hydroperoxide-induced TBA-reactive substances in erythrocyte membrane ghosts. Therefore, the effects of various known antioxidative compounds on the ileal ulcer formation induced by BFMeT were investigated. While α-tocopherol, t-butyl-1, 4-hydroxyanisole and allopurinol did not repress ulcer formation after BFMeT treatment, ascorbic acid, dimethyl sulfoxide, glutathione and β-carotene significantly inhibited formation. Among these compounds, ascorbic acid was the most effective. Accumulation of TBA-reactive substances in the ileal mucosa after BFMeT treatment also decreased significantly in rats treated with ascorbic acid. In addition, the percentage of Gram-negative rods in the ileal contents of rats treated with BFMeT and tap water was dramatically increased, but it was not increased in rats treated with BFMeT and these antioxidants. A positive correlation between the percentage of Gram-negative rods and the number of ileal ulcers was also observed. These results suggest that lipid peroxidation mediated by oxygen radicals plays an important role in the induction of ileal ulcers by BFMeT in rats, and that lipopolysaccharide-activated neutrophils probably produce highly reactive hypochlorous acid and hydrogen peroxide, which are inactivated by ascorbic acid and glutathione, respectively.

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