Multiplication of <i>Legionella pneumophila</i> in HeLa Cells in the Presence of Cytoskeleton and Metabolic Inhibitors

• Goldoni Paola

  Istituto di Microbiologia, Università "La Sapienza"

• Cattani Lorena

  Istituto di Microbiologia, Università "La Sapienza"

• Carrara Stefania

  Istituto di Microbiologia, Università "La Sapienza"

• Pastoris Maddalena Castellani

  Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanità

• Sinibaldi Laura

  Istituto di Microbiologia, Università "La Sapienza"

• Orsi Nicola

  Istituto di Microbiologia, Università "La Sapienza"

抄録

A study has been carried out on the action of cytoskeleton and metabolic inhibitors on intracellular multiplication in HeLa cells of a virulent strain of Legionella pneumophila serogroup 6. The effects of the substances were separately tested on both penetration and intracellular multiplication of L. pneumophila. Only cytochalasin A and 2-deoxy-n-glucose (2dG) affected bacterial internalisation, whereas intracellular multiplication was inhibited by cytochalasins A, B, C, D and J (D being the most active) and by 2dG with a dose-response effect. The action of 2dG was counteracted by 50mM glucose. Experiments carried out with cytochalasin D and a rhodamine-phalloidin conjugate showed the involvement of cytoskeletal elements in intracellular multiplication of Legionella; compounds acting on microtubules had no effect.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 42 (4), 271-279, 1998

  Center For Academic Publications Japan

詳細情報

• CRID: 1573387452895159680
• NII論文ID: 130003484595
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00084783639?from=CiNii
• 本文言語コード: en
• データソース種別:
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