Studies on Rabies Virus RNA Polymerase
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- Morimoto Kinjiro
- Department of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Kyoto University
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- Akamine Takayuki
- Department of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Kyoto University
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- Takamatsu Fumihiko
- Department of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Kyoto University
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- Kawai Akihiko
- Department of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Kyoto University
抄録
To investigate the RNA polymerase of rabies virus, we cloned a cDNA of the catalytic subunit (called L protein because of its large molecular size) of the HEP-Flury strain, an avirulent strain obtained by high frequencies of serial embryonated hen egg passages. Nucleotide sequencing showed that the cDNA encodes a long polypeptide of 2, 127 amino acids (Mr. 242, 938). A comparison of the deduced amino acid sequence with that of other strains (PV and SAD B19) indicated that the sequence was highly conserved, except for several amino acid substitutions which were accumulated in some limited regions. A fragment of the cDNA was used for expression in Escherichia coli (E. coli) to prepare the L antigen for raising the antibodies in rabbits. Immunoprecipitation studies with the rabbit antiserum showed that the polypeptides produced in the L cDNA-transfected COS-7 cells displayed almost the same electrophoretic mobility as that of authentic L protein. Immunofluorescence studies indicated that both L and P (another subunit of RNA polymerase) proteins displayed colocalized distribution with the nucleocapsid antigen (N) in the cytoplasmic inclusion bodies, where envelope proteins (G and M) were absent. On the other hand, expression of the L protein alone did not cause inclusion body-like granular distribution, suggesting that the inclusion body-like accumulation depends on certain interaction(s) with other viral gene products, probably with the ribonucleoproteins comprising the inclusion bodies.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 42 (7), 485-496, 1998
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1570291228151993472
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- NII論文ID
- 130003484618
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles