Genotypic and Phenotypic Analysis of Bromovirus Adaptive Mutants Derived from a Single Plant
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- Fujita Yasunari
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
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- Mise Kazuyuki
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
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- Furusawa Iwao
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
Abstract
Eight adaptive mutant clones have been made from the total RNA extracted from uninoculated upper leaves of a single cowpea plant exhibiting systemic infection after inoculation with a hybrid cowpea chlorotic mottle bromovirus (CCMV) with the 3a movement protein gene of CCMV replaced by that of cowpea-nonadapted brome mosaic bromovirus (BMV). Sequence and mutational analyses of these clones showed genotypic and phenotypic diversity of the cloned virus population, but all examined clones had the adaptive mutation, A to C at position 776 within the BMV 3a gene, required for the systemic infection of cowpea. The data support the quasispecies model for RNA virus population, and suggest that the maintenance of the adaptive mutation may be due to powerful selection pressure in an infection process.
Journal
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- Japanese Journal of Microbiology
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Japanese Journal of Microbiology 43 (2), 181-185, 1999
Center For Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1570572703128695296
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- NII Article ID
- 130003484682
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- ISSN
- 03855600
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- Text Lang
- en
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- Data Source
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- CiNii Articles