Molecular Cloning and Characterization of the <i>oprQ</i> Gene Coding for Outer Membrane Protein OprE3 of <i>Pseudomonas aeruginosa</i>

• Okamoto Kiyomi

  Department of Microbiology, Kyoto Pharmaceutical University

• Gotoh Naomasa

  Department of Microbiology, Kyoto Pharmaceutical University

• Tsujimoto Hideto

  Department of Microbiology, Kyoto Pharmaceutical University

• Yamada Hiroshi

  Department of Microbiology, Kyoto Pharmaceutical University

• Yoshihara Eisaku

  Department of Molecular Life Science, School of Medicine, Tokai University

• Nakae Taiji

  Department of Molecular Life Science, School of Medicine, Tokai University

• Nishino Takeshi

  Department of Microbiology, Kyoto Pharmaceutical University

抄録

We cloned and characterized the oprQ gene coding for outer membrane protein OprE3 of Pseudomonas aeruginosa PAO1. The oprQ gene was composed of 1, 275 base pairs including a sequence encoding for the signal sequence and a mature protein with a Mr of 44, 602. Computer-aided alignment and hydropathy analyses of the predicted amino acid sequences suggested that OprE3 is a transmembrane protein homologous to outer membrane proteins of P. aeruginosa such as OprD2 (OprD) porin and OprE1 (OprE) porin. Susceptibility to several antibiotics of the strains lacking or overproducing OprE3 was indistinguishable from that of the wild-type strain, suggesting that OprE3 is unlikely involved in the diffusion of carbapenems and other β-lactam antibiotics.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 43 (3), 297-301, 1999

  Center For Academic Publications Japan

詳細情報

• CRID: 1574231877825936384
• NII論文ID: 130003484698
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00086023047?from=CiNii
• 本文言語コード: en
• データソース種別:
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