Genetic Variation in the 5' End and NS5B Regions of Classical Swine Fever Virus Genome among Japanese Isolates

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  • Harasawa Ryo
    Animal Center for Biomedical Research, Faculty of Medicine, The University of Tokyo
  • Giangaspero Massimo
    Institute of Special Pathology and Veterinary Medical Clinic, Faculty of Veterinary Medicine, The University of Milan

抄録

Sixteen clinical strains of classical swine fever virus (CSFV) isolated in Japan were subjected to analyses of nucleotide sequence variations in the 5' end and NS5B regions of the genome. These isolates were divided into three genovars, CSFV-1, CSFV-2 and CSFV-3, based on palindromic nucleotide substitutions at the three variable loci in the 5' untranslated region (UTR). Phylogenetic trees constructed from nucleotide sequences in the 5'-UTR and NS5B gene indicated that the CSFV strains were divided into three clusters, I, II and III. CSFV strains included in clusters I, II and III were identical to those in the CSFV-1, CSFV-2 and CSFV-3 genovars, respectively.

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