Cloning, Sequencing, and Functional Expression in <i>Escherichia coli</i> of Chaperonin (<i>groESL</i>) Genes from <i>Vibrio cholerae</i>
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- Mizunoe Yoshimitsu
- Department of Bacteriology, Kyushu University
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- Wai Sun Nyunt
- Department of Bacteriology, Kyushu University
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- Umene Kenichi
- Department of Virology, Faculty of Medicine, Kyushu University
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- Kokubo Takayuki
- Department of Bacteriology, Kyushu University
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- Kawabata Shun-ichiro
- Department of Biology, Faculty of Science, Kyushu University
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- Yoshida Shin-ichi
- Department of Bacteriology, Kyushu University
抄録
Using a series of oligonucleotides synthesized on the basis of conserved nucleotide motifs in heat-shock genes, the groESL heat-shock operon from a Vibrio cholerae TSI-4 strain has been cloned and sequenced, revealing that the presence of two open reading frames (ORFs) of 291 nucleotides and 1, 632 nucleotides separated by 54 nucleotides. The first ORF encoded a polypeptide of 97 amino acids, GroES homologue, and the second ORF encoded a polypeptide of 544 amino acids, GroEL homologue. A comparison of the deduced amino acid sequences revealed that the primary structures of the V. cholerae GroES and GroEL proteins showed significant homology with those of the GroES and GroEL proteins of other bacteria. Complementation experiments were performed using Escherichia coli groE mutants which have the temperature-sensitive growth phenotype. The results showed that the groES and groEL from V. cholerae were expressed in E. coli, and groE mutants harboring V. cholerae groESL genes regained growth ability at high temperature. The evolutionary analysis indicates a closer relationship between V. cholerae chaperonins and those of the Haemophilus and Yersinia species.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 43 (6), 513-520, 1999
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1573105977917865728
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- NII論文ID
- 130003484727
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles