Characterization of the <i>Coxiella burnetii suc</i>B Gene Encoding an Immunogenic Dihydrolipoamide Succinyltransferase

J-STAGE
  • Nguyen Sa VAN
    Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University
  • To Ho
    Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University
  • Yamaguchi Tsuyoshi
    Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University
  • Fukushi Hideto
    Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University
  • Hirai Katsuya
    Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University

抄録

The Coxiella burnetii sucB gene encoding the dihydrolipoamide succinyltransferase (E2o) enzyme was cloned by immunological screening of a λEMBL3 genomic library prepared from strain Nine Mile DNA and sequenced. The homology of the cloned gene product to the counterpart in Escherichia coli was 54.3%, but the homology of the N-terminal region was only 42%. The gene was expressed in E. coli as an independent unit from its own promoter, producing an immunoreactive protein of about 50kDa on SDS-PAGE which reacted with antisera from laboratory animals and sera from human patients with acute Q fever. The study results suggest that the C. burnetii E2o enzyme may serve as a potential target antigen for diagnostic assays for Q fever.

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