A Simple and Rapid Method for Purifying Staphylococcal Exfoliative Toxin A

• Yamazaki Taisuke

  Department of Molecular Genetics, Kohno Clinical Medicine Research Institute

• Sakurai Susumu

  Department of Molecular Genetics, Kohno Clinical Medicine Research Institute

• Matsuo Naruto

  Department of Pharmacology of Kohno Clinical Medicine Research Institute

• Machida Katsuhiko

  Department of Laboratory Medicine, The Jikei University School of Medicine

• Kohno Minoru

  Kohno Clinical Medicine Research Institute

抄録

A rapid and efficient method of purification of staphylococcal exfoliative toxin A, the causative agent of staphylococcal scalded skin syndrome (SSSS), has been developed. It is based on ammonium sulfate precipitation of the culture supernatant and hydrophobic interaction chromatography on Phenyl-Sepharose CL-4B. This procedure results in 87-fold purification of this toxin, which appears as a single band in sodium dodecyl sulfate-polyacrylamide gels.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 44 (12), 1067-1070, 2000

  Center For Academic Publications Japan

詳細情報

• CRID: 1571698603034625920
• NII論文ID: 130003484818
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00089729342?from=CiNii
• 本文言語コード: en
• データソース種別:
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