Cell Surface-Associated Enolase in <i>Actinobacillus</i> <i>actinomycetemcomitans</i>

J-STAGE
  • Hara Hiroaki
    Department of Periodontology and Endodontology, Okayama University Dental School
  • Ohta Hiroyuki
    Department of Microbiology, Okayama University Dental School
  • Inoue Tetsuyoshi
    Department of Microbiology, Okayama University Dental School
  • Ohashi Toshio
    Department of Periodontology and Endodontology, Okayama University Dental School
  • Takashiba Shogo
    Department of Periodontology and Endodontology, Okayama University Dental School
  • Murayama Yoji
    Department of Periodontology and Endodontology, Okayama University Dental School
  • Fukui Kazuhiro
    Department of Microbiology, Okayama University Dental School

抄録

Cell surface-associated materials of Actinobacillus actinomycetemcomitans were extracted by a short incubation of the cell suspension in a Tris-buffered saline in the presence and absence of a restriction enzyme, EcoRI. The supernatants (which we termed EcoRI extract and surface extract, respectively) contained a number of extracellularly released proteins. Of these proteins, four major proteins were identified by N-terminal sequencing to be the 34 and 39kDa outer membrane proteins, the GroEL-like protein, and a 47kDa protein homologous to Haemophilus influenzae enolase. Enolase activity was found in the extracts and its relative amount of activity in the EcoRI extract from a culture of the mid-exponential growth phase was estimated as 5.7% of total enzyme activity. In contrast, the relative amount of activity of another cytosolic enzyme, lactate dehydrogenase, was extremely low in the extracts and also in the culture supernatant. These results suggest the external localization of enolase in this bacterium.

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