Isolation and Characterization of a Major Antigenic Component of <i>Malassezia globosa</i> to IgE Antibodies in Sera of Patients with Atopic Dermatitis
-
- Koyama Tomoki
- Department of Dermatology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine
-
- Kanbe Toshio
- Laboratory of Medical Mycology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine
-
- Ishiguro Ayako
- Department of Nursing, Nagoya University School of Health Sciences
-
- Kikuchi Akihiko
- Laboratory of Medical Mycology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine
-
- Tomita Yasushi
- Department of Dermatology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine
抄録
Three major components of Malassezia globosa were isolated from 2-ME extracts of this fungus by ion-exchange column chromatography and are referred to as Malg46a, Malg46b and Malg67, respectively. IgE antibodies to these components in the sera of patients with AD were detected by immunoblots. In Western blot, IgE antibodies to Malg46b were most frequently detected in the sera of AD patients. Dot blot with the Malg46b-containing fraction immunologically reacted with 69% of the sera of the patients, and with 83% of the sera of the patients who were positive for IgE antibodies to the 2-ME extract of M. globosa in the Western blot. The intensities generated for each dot correlated well with the total intensities generated for the 2-ME extract of M. globosa in the Western blot (r=0.763). In the lectin blot, Con A reacted with both Malg46a and Malg46b but not with Malg67. The polyclonal antibody to Malg46b reacted strongly only with the 2-ME extract of M. globosa and reacted slightly with M. restricta. In conclusion, a glycoprotein, Malg46b of M. globosa, is dominantly expressed in this fungus and is a possible major antigen for IgE antibodies in patients with AD.
収録刊行物
-
- MICROBIOLOGY and IMMUNOLOGY
-
MICROBIOLOGY and IMMUNOLOGY 44 (5), 373-379, 2000
Center For Academic Publications Japan
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1572824502941455616
-
- NII論文ID
- 130003484860
-
- ISSN
- 03855600
-
- 本文言語コード
- en
-
- データソース種別
-
- CiNii Articles