Determination of the Protective Effects of Neutralizing Anti-Hepatitis B Virus (HBV) Immunoglobulins by Epitope Mapping with Recombinant HBV Surface-Antigen Proteins
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- Park Jung-Hyun
- Protein Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology
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- Cho Eun-Wie
- Protein Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology
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- Lee Yun-Jung
- Protein Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology
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- Shin Song Yub
- Protein Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology
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- Kim Kil Lyong
- Protein Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology
抄録
Anti-hepatitis B virus (HBV) surface-antigen immunoglobulins prepared from human sera are clinical reagents which have been approved for prophylactic treatment in HBV-exposed persons. The passive immunoprophylaxis with immunoglobulins is meant to cross-link viral particles, which are then further cleared by the host's own immune system. While antibodies specific for both anti-S- and anti-preS proteins have been proved to serve as effective anti-viral agents, so far the fine antigen specificity of clinical immunoglobulin preparations has not been determined. Using recombinant proteins covering the hepatitis B surface antigen, in the present study, the specificity of a commercially available immunoglobulin preparation was determined and immunodominant epitopes were mapped. Here, it is shown that the major reactivity of anti-HBV immunoglobulins is directed against the S-protein, and that no reactivity to the preS2 but a weak binding activity to the preS1 region was detectable. The antigen reactivity within the preS1 region was biased to the C-terminal region, which indicates the presence of a putative B-cell epitope. The evaluation of the antigen specificity and determination of novel protective epitopes will provide valuable information for the further development and improvement of prophylactic HBV immunoglobulins.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 44 (8), 703-710, 2000
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1573668927871593088
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- NII論文ID
- 130003484895
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles