Guanosine Diphosphate Mannose in Conformational Relation to Other Suger Nucleotides and Phosphates in Solution
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Molecular conformational transition of GDPMan and solution conformation of α-D-mannopyranose moiety in Man-1-P and GDPMan were examined in relation to other sugar nucleotides and phosphates. GDPMan and other sugar nucleotides examined revealed changes in the optical rotation in sigmoidal curve in water by addition of urea. The change was reversible without significant decomposition and is attributable to dissociation of an ordered form into a random form. Optical conformational values [<i>K</i>]<sup>21</sup><sub>D</sub> in 8M urea solution were +116° for GDPMan, +58°_??_+79° for UDPGlc, +79° for UDPGal, +135°_??_+143° for UDPGlcNAc, and +138°_??_+155° for UDPGIcA.<br> NMR analysis and periodate oxidation study revealed the <sup>4</sup><i>C</i><sub>1</sub> conformation of α-D-hexopyranose moieties in Man-1-P, Glc-1-P, GDPMan, UDPGlcNAc and UDPGalNAc.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 38(8), 1515-1520, 1974
Japan Society for Bioscience, Biotechnology, and Agrochemistry