N-terminal sequence of soybean .BETA.-amylase.

• MIKAMI Bunzo

  Research Institute for Food Science, Kyoto University

• NOMURA Keiichi

  Research Institute for Food Science, Kyoto University

• MORITA Yuhei

  Research Institute for Food Science, Kyoto University

抄録

The blocked N-terminus and N-terminal sequence of soybean β-amylase were determined by analyzing the acidic peptides derived on peptic digestion of the enzyme. The acidic peptides were separated from the digest on a Dowex 50×2 column and purified by reversed phase-high performance liquid chromatography (RP-HPLC). The major acidic peptide, Pep-4, was a heptapeptide with a molecular weight of 766. Forty-eight hundredths mol acetyl group and 0.61mol acetyl-Ala per mol of Pep-4 were detected on RP-HPLC analysis. The N-terminal 9 amino acid sequence of soybean β-amylase was deduced to be acetyl-Ala-Thr-Ser-Asp-Ser-Asn-Met-(Gly-Leu) from the results of sequence analysis of Pep-4 and amino acid analysis of other acidic peptides.

収録刊行物

• The Journal of Biochemistry

  The Journal of Biochemistry 100 (2), 513-516, 1986

  The Japanese Biochemical Society

詳細情報

• CRID: 1573668927917185408
• NII論文ID: 130003530049
• ISSN: 0021924X
• Web Site: https://jlc.jst.go.jp/DN/JALC/00171286746?from=CiNii
• 本文言語コード: en
• データソース種別:
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