Module-Specific Antibodies against Human Connective Tissue Growth Factor: Utility for Structural and Functional Analysis of the Factor as Related to Chondrocytes
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- Minato Masanao
- Department of Biochemistry and Molecular Dentistry Department of Orthodontics and Dentofacial Orthopedics, Okayama University Graduate School of Medicine and Dentistry
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- Kubota Satoshi
- Department of Biochemistry and Molecular Dentistry
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- Kawaki Harumi
- Department of Biochemistry and Molecular Dentistry
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- Nishida Takashi
- Department of Biochemistry and Molecular Dentistry
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- Miyauchi Akira
- Kazusa Laboratory, Protein Express Co. Ltd.
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- Hanagata Hiroshi
- Research Laboratory, Higeta Shoyu Co. Ltd.
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- Nakanishi Tohru
- Department of Biochemistry and Molecular Dentistry
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- Takano-Yamamoto Teruko
- Department of Orthodontics and Dentofacial Orthopedics, Okayama University Graduate School of Medicine and Dentistry
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- Takigawa Masaharu
- Kazusa Laboratory, Protein Express Co. Ltd.
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抄録
Connective tissue growth factor/hypertrophic chondrocyte specific gene product 24 (CTGF/Hcs24/CCN2) shows diverse functions in the process of endochondral ossification. It promotes not only the proliferation and differentiation of chondrocytes and osteoblasts in vitro, but also angiogenesis in vivo. The ctgf gene is a member of the gene family called CCN, and it encodes the characteristic 4-module structure of this family, with the protein containing IGFBP, VWC, TSP and CT modules. We raised several monoclonal antibodies and polyclonal antisera against CTGF, and located the epitopes in the modules by Western blotting. For mapping the epitopes, Brevibacillu-sproduced independent modules were utilized. As a result, at least 1 antibody or antiserum was prepared for the detection of each module in CTGF. Western blotting with these antibodies is expected to be useful for the analysis of CTGF fragmentation. Moreover, we examined the effects of these monoclonal antibodies on the biological functions of CTGF. One out of 3 humanized monoclonal antibodies was found to neutralize efficiently the stimulatory effect of CTGF on chondrocytic cell proliferation. This particular antibody bound to the CT module. In contrast, surprisingly, all of the 3 antibodies recognizing IGFBP, VWC and CT modules stimulated proteoglycan synthesis in chondrocytic cells. Together with previous findings, these results provide insight into the structural-functional relationships of CTGF in executing multiple functions.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 135 (3), 347-354, 2004
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679942154624
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- NII論文ID
- 130003534671
- 10016198813
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- NII書誌ID
- AA00694073
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- COI
- 1:CAS:528:DC%2BD2cXjvFKisLo%3D
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- ISSN
- 17562651
- 0021924X
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- NDL書誌ID
- 6904011
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- PubMed
- 15113833
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可