CYANIDE INSENSITIVE TERMINAL RESPIRATORY SYSTEM IN AEROBACTER CLOACAE
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1. <i>Aerobacter cloacae</i> isolated from soil was found to show a cyanide-insen-sitive growth adaptively.<br> 2. The glucose oxidation by cyanide adapted cells was cyanide insensi-tive, whereas that by cyanide non-adapted cells was remarkably inhibited by cyanide.<br> 3. The oxidation of DPNH and succinate by the cell-free extract pre-pared from both cyanide adapted and cyanide non-adapted cells, was cyanide insensitive. Almost all of the oxidase activity was localized in the particulate fraction.<br> 4. A cytochrome component which has absorption maxima at 557 and 530mμ was detected in particulate fraction and was found to be reduced by the addition of DPNH, succinate and dithionite.<br> 5. The physiological significance of this cyanide insensitive terminal oxidase system was discussed.<br> The authors wish to express their sincere appreciation to Mr. K. Komagata, The Institute of Applied Microbiology, University of Tokyo, for his kind advise in the diagnosis of bacteria.
- J Biochem (Tokyo)
J Biochem (Tokyo) 46(3), 373-381, 1959
The Japanese Biochemical Society