MECHANISM OF CYANIDE RESISTANCE IN ACHROMOBACTER:III. NATURE OF TERMINAL ELECTRON TRANSPORT SYSTEM AND ITS SENSITIVITY TO CYANIDE

J-STAGE
  • MIZUSHIMA SHÔJI
    Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo
  • ARIMA KEI
    Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo

Abstract

1. Water soluble preparation which catalyzes the electron transfer between DPNH or succinate and oxygen was obtained from subcellular particulate preparation of a strain of Achromobacter.<br> 2. Cytochrome b1 and a2 contained in the preparation were slowly reduced by succinate or DPNH and oxidized in an instant by oxygen.<br> 3. Cyanide (10-3M) remarkably but not completely inhibited the oxido-reduction of cytochrome a2 but did not inhibit the reduction of cytochrome b1.<br> 4. The direction of electron transport between succinate or DPNH and oxygen was postulated as follows;<br> 5. These results supported a view on the mechanism of cyanide resistance in this bacteria.<br> The authors wish to express their sincere appreciation to Dr. K. Sakaguchi for his encouragement and advice in this investigation, and are indebted to Dr. K. Okunuki Osaka University for his kind suggestion and discussion.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 47 (6), 837-845, 1960

    The Japanese Biochemical Society

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