Isolation and localization from chicken gizzard of an inhibitory protein for Mg2+-activated skeletal muscle actomyosin ATPase.
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1. An inhibitory protein for Mg<sup>2+</sup>-activated actomyosin ATPase from rabbit skeletal muscle was prepared from frozen chicken gizzard and purified by DEAE-Sephadex chromatography and gel filtration.<br>2. The inhibition by this protein was released by the addition of skeletal muscle troponin C and was independent of gizzard tropomyosin.<br>3. Localization of the inhibitory protein in gizzard muscle tissue and gizzard thin filaments was demonstrated by immunohistological techniques and immunodiffusion tests.
- J Biochem (Tokyo)
J Biochem (Tokyo) 84(4), 947-955, 1978
The Japanese Biochemical Society