Effects of Mushroom Toxins on Glycogenolysis; Comparison of Toxicity of Phalloidin, .ALPHA.-Amanitin and DL-Propargylglycine in Isolated Rat Hepatocytes.

• KAWAJI Akiko

• YAMAUCHI Kiyotaka

• FUJII Shinobu

• NATSUKI Reiko

• TAKABATAKE Eigo

• YAMAURA Yoshio

抄録

The effects of phalloidin and α-amanitin as toxins of Amanita species and DL-propargylglycine identified from A. abrupta on the glycogenolysis in isolated rat hepatocytes were investigated. Phalloidin decreased glycogen content and activated phosphorylase α activity remarkably. α-Amanitin also decreased glycogen content significantly but activated phosphorylase α activity slightly. DL-Propargylglycine slightly affected glycogenolysis. Phalloidin, which most affected glycogenolysis among the three compounds mentioned above, elevated cytosolic Ca²⁺ concentration ([Ca²⁺]_i) and ⁴⁵Ca uptake into cells. Phalloidin depressed slightly ³H-inositol incorporation into phosphatidylinositol (PI) and remarkably phosphatidylinosito 4, 5-bisphosphate (PIP₂) but increased phosphoinositides breakdown. These results suggest that phalloidin alters phosphoinositides turnover and intracellular Ca²⁺ homeostasis, subsequently activates phosphorylase α, resulting in glycogenolysis in isolated rat hepatocytes.

収録刊行物

• Journal of Pharmacobio-Dynamics

  Journal of Pharmacobio-Dynamics 15 (3), 107-112, 1992

  公益社団法人 日本薬学会

キーワード

• DL-propargylglycine

詳細情報

• CRID: 1390282679599068672
• NII論文ID: 130003544213
• DOI: 10.1248/bpb1978.15.107
• ISSN: 18811353
• ISSN: 0386846X
• PubMed: 1320679
• Web Site: http://www.jstage.jst.go.jp/article/bpb1978/15/3/15_3_107/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可