Evidence for the Presence Keratinocyte Growth Factor(KGF) in Human Ovarian Follicles.

• OSUGA YUTAKA

  Department of Obstetrics and Gynecology, University of Tokyo

• KOGA KAORI

  Department of Obstetrics and Gynecology, University of Tokyo

• TSUTSUMI OSAMU

  Department of Obstetrics and Gynecology, University of Tokyo CREST, Japan Science and Technology, Kawaguchi Center Building

• YANO TETSU

  Department of Obstetrics and Gynecology, University of Tokyo

• KUGU KOJI

  Department of Obstetrics and Gynecology, University of Tokyo

• MOMOEDA MIKIO

  Department of Obstetrics and Gynecology, University of Tokyo

• OKAGAKI RYUGO

  Department of Obstetrics and Gynecology, University of Tokyo

• SUENAGA AKIHIKO

  Department of Obstetrics and Gynecology, University of Tokyo

• FUJIWARA TOSHIHIRO

  Department of Obstetrics and Gynecology, University of Tokyo

• FUJIMOTO AKIHISA

  Department of Obstetrics and Gynecology, University of Tokyo

• MATSUMI HIROTAKA

  Department of Obstetrics and Gynecology, University of Tokyo

• HIROI HISAHIKO

  Department of Obstetrics and Gynecology, University of Tokyo

• TAKETANI YUJI

  Department of Obstetrics and Gynecology, University of Tokyo

抄録

The presence of keratinocyte growth factor (KGF) in human follicular fluid (FF) was investigated in a total of 145 FFs obtained during oocyte retrieval for in vitro fertilization (IVF) from 29 patients with no apparent endocrine disorders. The concentrations of KGF, estradiol, progesterone, testosterone and human chorionic gonadotropin (hCG) in FF were measured by enzyme-linked immunosorbent assay. FF samples contained relatively higher amounts of KGF (2194±87pg/ml), whereas its concentrations in serum were below assay limit (<31.2pg/ml). Concentrations of KGF in FF were positively correlated with both progesterone (r=0.311, p<0.0005) and testosterone (r=0.230, p<0.01) concentrations in FF. However, KGF concentrations were not ignificantly correlated with estradiol and hCG concentrations. KGF in FF was detected as a broad band (26-29kD) by immunoblotting, the size being reduced by 7kD after N-glycosidase treatment. In an in vitro experiment, KGF suppressed the basal and hCG-stimulated progesterone production by cultured human luteinized granulosa cells. In summary, we demonstrated the presence of KGF in human ovarian follicles, suggesting its possible role as a local<br>factor in regulating human ovarian functions.

収録刊行物

• Endocrine Journal

  Endocrine Journal 48 (2), 161-166, 2001

  一般社団法人 日本内分泌学会

キーワード

• Ovary
• Keratinocyte growth factor
• Follicular fluid
• IVF-ET

詳細情報

• CRID: 1390001206300412416
• NII論文ID: 130003550145
• NII論文ID: 50000886843
• NII書誌ID: AA10901436
• DOI: 10.1507/endocrj.48.161
• COI: 1:CAS:528:DC%2BD3MXntFKktrg%3D
• ISSN: 13484540
• ISSN: 09188959
• PubMed: 11456262
• Web Site: http://www.jstage.jst.go.jp/article/endocrj1993/48/2/48_2_161/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2002001172
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可