条件的不死化細胞株を用いた脳関門機能の解明

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書誌事項

タイトル別名
  • FUNCTIONAL ANALYSIS OF THE BLOOD BRAIN BARRIER USING CONDITIONALLY IMMORTALIZED CELL LINES

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抄録

In vitro cell culture system is one of useful tools for analyzing function of the blood brain barrier (BBB). We have established conditionally immortalized endothelial cell lines from the transgenic rat and mouse harboring temperature sensitive SV40 large T antigen gene. These culture cells expressed GLUT-1 and mdrIa and their growth depended on the expression of is SV40 T antigen in cells. Using these cell lines together with in vivo analyzing system such as brain efflux index method, we revealed following new BBB functions: 1. Amino acid transport involved in osmoregulation. 2. Stereoselective acidic amino acid transport. 3. Efflux transport system of neurotransmitter, GABA. 4. Efflux transport system of neurotransmitter metabolites, homovanillic acid, and effect of uremic toxin on the efflux system. 5. Supply creatine as energy depot to brain. These evidences suggested that the BBB is not only a supporting system but also a protecting system for maintaining the central nervous system.

収録刊行物

  • 薬物動態

    薬物動態 16 (supplement), 110-111, 2001

    日本薬物動態学会

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