豚丹毒菌2型株の増殖性と免疫学的性状 Growth Ability and Immunological Properties of Erysipelothrix rhusiopathiae Serotype 2.

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Abstract

血清型2型の豚丹毒菌5株の液体培地における増殖性, 全培養菌液で調製した不活化ワクチンのマウスにおける免疫原性, 菌体表層蛋白のSDS-PAGEプロファイル, およびそのイムノブロット像を比較したところ, 多摩-96株は安定した増殖性を示し, 免疫原性が最も高かった. 菌体表層蛋白のSDS-PAGE像とイムノブロット像は供試株間でほぼ類似し, 66-64kDa蛋白が主要共通抗原として認められた.

Five field strains of Erysipelothrix rhusiopathiae belonging to serotype 2 were compared for their growth ability, immunogenicity in mice, SDS-PAGE profile of cell surface proteins and their immunoblotting patterns. Strain Tama-96 showed the most stable growth in Feist medium and tryptose phosphate broth with Tween 80 (TPB), and its immunogenicity was highest in a mouse protection test using the inactivated vaccines prepared from 20-h TPB culture. The 50% mouse protective dose of the vaccine was only 12 μl. SDS-PAGE and immunoblotting patterns of the proteins were similar among the strains in general and indicated that 66 to 64 kDa protein antigens were dominant.

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 58(1), 87-90, 1996

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130003572259
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • Data Source
    J-STAGE 
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