バルビタール・リチウム・塩酸緩衝液による血清Ｃ３成分のＩｍｍｕｎｏｆｉｘａｔｉｏｎ　Ｅｌｅｃｔｒｏｐｈｏｒｅｔｏｇｒａｍ

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  • Immunofixation electrophoretogram of the third component of complement in human serum by the use of new barbital·lithium·HCl buffer on agarose gel

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Separation and measurement of the third component of complement (C3) and its conversion products in normal human serum can be achieved using the immunofixation electrophoretic technique on agarose gel with the barbltal·lithium·HCl buffer (pH 8.6, μ0.1) firstly proposed by M. Ichimura and T. Baba.<br>The C3 immunofixation electrophoresis worked well, because utilization of this new buffer was effective on highly clear separation of the β-positioned serum proteins. Consequently, we have resolved native C3 and six C3 conversion products and simultaneously obtained the C3 conversion sequence in serum.<br>In addition, we are able to measure the concentrations of serum C3 on its conversion products and the C3 biological activities by means of the immunofixation electrophoresis.

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