高解像度ラージゲルを用いた二次元電気泳動法による細胞内発現タンパク質の網羅的検出 [in Japanese] Multiple Large Gel Two-dimensional Electrophoresis for Proteomics. [in Japanese]
Access this Article
A fundamental concern about proteomics is improving the coverage of detection of a cellular proteome using our technology. In the case of two-dimensional gel electrophoresis (2-DE), a core technology of proteomics, enlargement of the gel size appears a straightforward and effective strategy for increasing the number of detected proteins. We utilized fourteen large 2-DE gels (twelve 24cm×7cm gels) which were assembled into a 93cm×103cm cybergel. Our data suggested that the cyber gel can display more than 11, 000 protein spots expressed in a 1-10<sup>5</sup> dynamic range in cells.
- SEIBUTSU BUTSURI KAGAKU
SEIBUTSU BUTSURI KAGAKU 47(1/2), 27-31, 2003
Japanese Electrophoresis Society