高解像度ラージゲルを用いた二次元電気泳動法による細胞内発現タンパク質の網羅的検出  [in Japanese] Multiple Large Gel Two-dimensional Electrophoresis for Proteomics.  [in Japanese]

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Author(s)

    • 野村 英子 Nomura Eiko
    • 奈良先端科学技術大学院大学 Division of Signal Transduction, Nara Institute of Science and Technology
    • 稲垣 直之 Inagaki Naoyuki
    • 奈良先端科学技術大学院大学|Recognition and Formation, PRESTO, JST Division of Signal Transduction, Nara Institute of Science and Technology|Recognition and Formation, PRESTO, JST

Abstract

A fundamental concern about proteomics is improving the coverage of detection of a cellular proteome using our technology. In the case of two-dimensional gel electrophoresis (2-DE), a core technology of proteomics, enlargement of the gel size appears a straightforward and effective strategy for increasing the number of detected proteins. We utilized fourteen large 2-DE gels (twelve 24cm×7cm gels) which were assembled into a 93cm×103cm cybergel. Our data suggested that the cyber gel can display more than 11, 000 protein spots expressed in a 1-10<sup>5</sup> dynamic range in cells.

Journal

  • SEIBUTSU BUTSURI KAGAKU

    SEIBUTSU BUTSURI KAGAKU 47(1/2), 27-31, 2003

    Japanese Electrophoresis Society

Codes

  • NII Article ID (NAID)
    130003607295
  • Text Lang
    JPN
  • ISSN
    0031-9082
  • Data Source
    J-STAGE 
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