Methylation of hepatitis B virus DNA and liver-specific suppression of RNA production in transgenic mouse.
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- YAMAMURA Ken-ichi
- Department of Medicine and Geriatrics, Osaka University Medical School
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- TSURIMOTO Toshiki
- Institute for Molecular and Cellular Biology, Osaka University
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- EBIHARA Taeko
- Department of Medicine and Geriatrics, Osaka University Medical School
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- KAMINO Kouzin
- Department of Medicine and Geriatrics, Osaka University Medical School
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- FUJIYAMA Asao
- Institute for Molecular and Cellular Biology, Osaka University
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- OCHIYA Takahiro
- Institute for Molecular and Cellular Biology, Osaka University
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- MATSUBARA Kenichi
- Institute for Molecular and Cellular Biology, Osaka University
抄録
Three lines of transgenic mice carrying hepatitis B virus (HBV) DNA were produced. In a pSHB1BB transgenic mouse carrying one copy of BamHI fragment of HBV with a deletion of BglII-BglII fragment, no RNA transcription was observed. Transgenic mice that had integrated either one or three tandem copies of HBV DNA produced RNA in various tissues except liver. However, neither initiation nor termination of transcription occurred at the correct place. Hepatitis B surface antigen and hepatitis B e antigen were not detectable in these transgenic mice. HBV DNA was methylated at all CCGG sequences, which might have resulted in aberrant RNA production. In two out of five transgenic mice HBV DNA was integrated into the Y chromosome. Although the frequency is unusually high, the mechanism is not known yet.
収録刊行物
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- Japanese Journal of Cancer Research GANN
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Japanese Journal of Cancer Research GANN 78 (7), 681-688, 1987
The Japanese Cancer Association
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詳細情報
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- CRID
- 1571417128151978112
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- NII論文ID
- 130003609000
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- ISSN
- 09105050
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles