ヒトリンパろ胞はい中心における血液凝固系及び線溶系因子の局在

• 山川 光徳

  山形大学医学部第二病理

• 田嶋 克史

  山形大学医学部第二病理

• 高木 理彰

  山形大学医学部第二病理

• 大江 信哉

  山形大学医学部第二病理

• 小山内 俊久

  山形大学医学部第二病理

• 工藤 俊

  山形大学医学部第二病理

• 伊藤 正尚

  山形大学医学部第二病理

• 佐藤 隆司

  山形大学医学部第二病理

• 今井 大

  山形大学医学部第二病理

書誌事項

タイトル別名

• Localization of blood coagulation- and fibrinolysis-factors in lymphoid germinal centers.

抄録

An immunohistochemical study of lymphoid germinal centers (GCs) with thirteen monospecific antibodies directed against blood coagulation-and fibrinolysis-factors was carried out using human reactive lymph node and tonsil tissues. The trypsinization of formalin-fixed, paraffin sections was required to demonstrate a dendritic immunoreaction within GCs. An optimal condition was under 0.1% concentration of trypsin, 20min, at 37°C. Frequent dendritic immunoreactions of factor XII, high molecular weight kininogen, factor X, factor V, and factor II, and often same immunoreactions of kallikrein, factor IX, factor XIIIs, and plasminogen were clearly found within GCs, while no dendritic reaction of factor VIII, factor I, factor XIIIa, and tissue-plasminogen activator (t-PA) was found. However, fresh frozen-and periodate-lysine-paraformaldehyde (PLP) fixed-cryostat sections revealed distinct dendritic immunostains within GCs of factor XIIIa and t-PA as well as other nine factors except for factor VIII and factor I. The immunostain on fresh frozen-cryostat sections was superior to that on PLP fixed-ones. The dendritic reaction was mostly expressed in a light zone within GCs. Furthermore, an immunoelectron microscopic observation was carried out using the PLP-fixed tonsil tissues. The immunoreaction of factor X and factor XIIIa was observed on the cell surface of lymphocytes, macrophages, and follicular dendritic cells (FDCs), and in the intercellular space within GCs, especially marked on the labyrinthine-like cell surface of the FDC's cytoplasmic extension. In addition, the immunoreaction was found on the cell surface of the fibroblastic reticulum cell in the mantle zone. Often the band-like localization of factor XIIIa was found in the subendothelium of blood capillaries within GCs. However, no reaction was found in the perinuclear cystern and rough endoplasmic reticulum in any cells within the GC. It was concluded that blood coagulation-and fibrinolysis-factors as well as complement components might be closely related to the GC-function.

収録刊行物

• 日本網内系学会会誌

  日本網内系学会会誌 29 (4), 383-392, 1989

  日本リンパ網内系学会

キーワード

• Lymphoid germinal center
• Blood coagulation
• Fibrinolysis
• Immunohistochemistry

詳細情報

• CRID: 1390282679676919808
• NII論文ID: 130003642086
• DOI: 10.3960/jslrt1961.29.383
• ISSN: 18836801
• ISSN: 03869725
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可