血小板結合免疫グロブリンに関する研究

• 小河原 はつ江

  群馬大学医療技術短期大学部

書誌事項

タイトル別名

• STUDIES ON PLATELET-ASSOCIATED IMMUNOGLOBULINS
• 第1報 PAIgG, PAIgM, PAIgA, PAC3測定の臨床的意義について 第2報 免疫性血小板減少症における対応抗原の検討

抄録

The amounts of platelet-associated immunoglobulins (PAIgG, PAIgA, PAIgM) and complement (PAC 3) were determined in patients with various types of thrombocytopenia including, idiopathic thrombocytopenic purpura (ITP). The mean amounts of PAIgG, PAIgA, PAIgM and PAC 3 were increased not only in patients with immune thrombocytopenia, but also in those with other types of thrombocytopenia. The percentage of patients with normal amounts of PAIgG, PAIgM, and PAC 3 was significantly lower among those with ITP than those with systemic lupus erythematosus, lymphoproliferative disorders or liver cirrhosis. In ITP, the mean peripheral blood platelet count (p <0.05) and platelet life-span (p<0.01) were significantly lower in patients with simultaneously increased amounts only of PAIgG, PAIgM and PAC 3 than in those with an increased amount only of PAIgG. In patients with ITP, the amounts of PAIgG, PAIgM and PAC 3 were decreased in those whose peripheral platelet count tended to increase after prednisolone treatment. These findings were useful for evaluating the efficacy of prednisolone. In patients receiving multiple platelet transfusion, the amounts of PAIgG, PAIgM, PAIgA and PAC 3 were significantly higher than normal, when peripheral platelet counts remained lower than 100 × 10⁹/L after the transfusion. Moreover, the PAIgG level showed a tendency to be high in proportion to the amount of platelet transfusions.<BR>Using SDS-PAGE and Western immunoblotting, the target antigens of blood platelets against anti-platelet serum antibodies were investigated in patients with idiopathic thrombocytopenic purpura (ITP), systemic lupus erythematosus (SLE) with thrombocytopenia, and multiple platelet-transfusion. No clear differences were found in the distribution pattern of various bands, obtained by immunoblotting among the individual disease groups, and several bands were observed in many patients. Under non-reducing conditions, antibodies reacting to antigens corresponding to GPIb (Band-1) and GPIIb/IIIa (Band-2) were found to be nearly the same in the bands of the ITP, SLE and platelet-transfused groups. No clear differences were found in the distribution of various target platelet antigens reacting to anti-platelet serum antibodies among these groups. The same results were observed under reducing conditions. These findings suggest that no differences exist in the type or distribution pattern of target platelet antigens reacting to anti-platelet serum antibodies, by means of the immunoblotting method, among the various types of thrombocytopenia studied.

収録刊行物

• 北関東医学

  北関東医学 44 (2), 105-124, 1994

  北関東医学会

キーワード

• 特発性血小板減少性紫斑病 (ITP)
• ウエスターンプロット法 (Western immunoblotting)
• 抗血小板抗体対応抗原
• 全身性エリテマトーデス (SLE)
• 血小板輸血

詳細情報

• CRID: 1390282680308141696
• NII論文ID: 130003690585
• DOI: 10.2974/kmj1951.44.105
• ISSN: 18836135
• ISSN: 00231908
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可