A型ボツリヌス菌の自己融解に関する研究

DOI Web Site

書誌事項

タイトル別名
  • Studies on Autolysis of <I>Clostridium botulinum</I> Type A
  • ゼン キン ノ ジコ ユウカイ
  • I. Autolysis of Whole Cells
  • 第1報 全菌の自己融解

この論文をさがす

抄録

Cells of Clostridium botulinum type A strain 190 grown in thioglycolate medium (GYPT medium) autolysed after having reached a maximum growth. This strain was dissociated into large and small colony-forming types in semisolid media. The cells obtained from the large colony type autolysed more rapidly than those from small one. Washed cells harvested at logarithmic growth phase lysed in phosphate buffer at 37°C within 2-3 hours. Autolysis rose above pH 6.0 and was optimal near pH 7.0. The potential for autolysis reached a maximum toward the end of the logarithmic growth phase and thereafter the cells became resistant to autolysis. The autolytic activity was destreyed by heating the cells at 60°C for 10 minutes and was slightly affected by cysteine (10-2M), N-ethylmaleimide (10-2M) and mercaptoethanol (10-1M).<BR>During autolysis nitrogen, protein, nucleic acids, reducing sugars, amino sugars and botulinum toxin were released from the cells as the reduction of the turbidity in cell suspension occurred. Electron microscopic observations on the process of autolysis revealed that the partial lysis of walls occurred first at the end of the organism and the cytoplasmic contents were lost through such lesions. The lysis of the wall centripetally spread and finally the morphological entity of the wall was completely lost. From these findings it is suggested that the autolysis may be proceded by auto-digestion of the cell wall at the end of the organism.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 9 (5), 358-363, 1968

    公益社団法人 日本食品衛生学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ