ハプトグロビン欠損者検出のための簡便なＥＬＩＳＡ法の開発

DOI 被引用文献5件 参考文献10件

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  • A SIMPLE ELISA FOR THE DETECTION OF HAPTOGLOBIN DEFICIENCY

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Haptoglobin (Hp) deficiency is thought to be a risk factor for anaphylactic transfusion reactions in Japan. A simplified sandwich ELISA was developed as a practical method for detection of Hp deficiency. Patients' sera, control sera and standard Hps were added to the anti-human Hp rabbit polyclonal antibody-coated micro-plates without dilution and incubated for 10min. After washing, captured Hp was detected using horseradish peroxidase conjugated anti-human Hp goat anti-serum and TMB substrate. Hp deficiency was identified by comparing the intensity of color development of the test serum with that of the standard Hp. With this system, it was possible to detect Hp in serum at a lower limit of 3μg/dL and to detect patients with congenital Hp deficiency among patients in whom serum Hp concentrations were under the detection limit of 5mg/dL when measured by peak-rate nephelometry. It was also possible to detect deficiencies with the unaided eye.<br>In conclusion, this method was easy to perform and sufficiently sensitive to allow the detection of congenital Hp deficiency among patients with disease-induced hypohaptoglobinemia. It may therefore be useful in avoiding anaphylactic transfusion reactions in patients requiring multiple transfusions. It can also be readily applied to expand the future haptoglobin-deficient donor pool.

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