Recombinant human bone morphogenetic protein‐2(rhBMP‐2)の歯周組織に対する影響  [in Japanese] The effects of recombinant human bone morphogenetic protein-2(rhBMP-2) on periodontal tissues.  [in Japanese]

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Author(s)

    • 石川 烈 Ishikawa Isao
    • 東京医科歯科大学歯学部第二歯科保存学講座 (歯周治療学講座) Department of Periodontology, School of Dentistry, Tokyo Medical and Dental University
    • 吉田 直樹 Yoshida Naoki
    • 東京医科歯科大学歯学部第二歯科保存学講座 (歯周治療学講座) Department of Periodontology, School of Dentistry, Tokyo Medical and Dental University
    • 杉山 榮一 Sugiyama Eiichi
    • 東京医科歯科大学歯学部第二歯科保存学講座 (歯周治療学講座) Department of Periodontology, School of Dentistry, Tokyo Medical and Dental University
    • 木下 淳博 Kinoshita Atsuhiro
    • 東京医科歯科大学歯学部第二歯科保存学講座 (歯周治療学講座) Department of Periodontology, School of Dentistry, Tokyo Medical and Dental University
    • 小田 茂 Oda Shigeru
    • 東京医科歯科大学歯学部第二歯科保存学講座 (歯周治療学講座) Department of Periodontology, School of Dentistry, Tokyo Medical and Dental University

Abstract

In order to clarify whether bone morphogenetic protein (BMP) was effective and useful in periodontal regenerative therapy, three experiments were performed.<BR>(1) Recombinant human BMP-2 (rhBMP-2) was applied in the horizontal alveolar bone defects of beagle dogs. The carrier was complex of gelatin and polylactic acid polyglycolic acid copolymer. The carrier without rhBMP-2 was used as a control. Twelve weeks later, animals were sacrificed and histological observations were performed. Substantial amount of new bone, cementum, and periodontal ligament were regenerated in the rhBMP-2 group. In the control group, little periodontal regeneration was observed.<BR>(2) rhBMP-2 was implanted with three types of ceramic carriers (biphasic calcium phosphate, β-tricalcium phosphate, and hydroxyapatite) under the rat dorsal skin. In the BCP-BMP group, bone like tissue was formed in all 4 rats, and new bone tissue forming lamellae was observed in 2 of the 4 rats. The amounts of new bone were relatively smaller in the TCP-BMP and HAP-BMP groups. No bone like tissue was observed in any rats in the control groups.<BR>(3) Although 500 ng/ml rhBMP-2 were added to the culture wells of human periodontal ligaments' cells, no elevation of ALP activity of the cells was observed.<BR>These results suggest that rhBMP-2 will be a beneficial material in periodontal regenerative therapy.

Journal

  • Ensho

    Ensho 16(3), 181-185, 1996

    The Japanese Society of Inflammation and Regeneration

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