Fundamental Studies on Clinical Chemistry. X. A New Micro Method for the Colorimetric Determination of Urinary Dehydroepiandrosterone
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A new micro method for the colorimetric determination of dehydroepiandrosterone (DHEA) was studied and the standard procedure for the amounts of 0∼50μg. of the steroid was established. The principle of this method is based on the observation that DHEA in acetone is oxidized to androst-4-en-3, 6, 17-trione by the Kiliani's reagent and its p-nitrophenylhydrazone produced a stable color in an alkaline solution of dimethyl-formamide showing an absorption maximum at 590 mμ. By means of the combination of the solvolysis with sulfuric acid in ethyl acetate, the urinary DHEA may quite specifically be determined in the presence of other steroids.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 13 (4), 414-419, 1965
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679148980864
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- NII論文ID
- 110003619585
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- NII書誌ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- PubMed
- 14294834
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可