Extracellular Polysaccharide of Cladosporium herbarum Studies on Fungal Polysaccharide. XIII

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Major water-soluble extracellular polysaccharide of C. herbarum obtained by DEAE-cellulose column fractionation and zone electrophoresis using 1% Na2B4O7 of crude polysaccharide is a galactomannan, [α]25D+27°(c=1.0, H2O), which was composed of D-galactose and D-mannose=1.0 : 1.5. From the results of periodate oxidation, Smith-type degradation, and methylation studies showed that the polysaccharide has a highly branched structure and contains 1→2 main linkage of mannopyranose with 1→4 linked galactopyranose, and the glycan was branching at C4 position of mannose residue. The terminal residues are galactofranose and mannopyranose.

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