Fluorometric determination of ampicillin and aminobenzylpenicilloic acid in presence of pivampicillin in body fluids.

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In order to investigate the transfer of ampicillin and pivampicillin in biological systems, the sensitive separatory determination method of ampicillin and aminobenzyl-penicilloic acid in presence of pivampicillin was developed. This method is based on the separation of these substances utilizing their different distribution behavior between the aqueous phase and the chloroform phase, followed by fluorometric determination. In the transfered studies in biological systems, marked differences were found in the rate of absorption and the amount of excretion between ampicillin and pivampicillin. Biotrans-formational studies were also discussed.

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