Interactions of Sepharose-Bound Neurophysin I and II with Oxytocin and Vasopressin

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The interactions of Sepharose-bound bovine neurophysin I and II with oxytocin and arginine-vasopressin were studied. The amounts of hormones bound to immobilized neurophysin II were found to depend strongly on temperature, being larger at 4° than at 36°. It was found that oxytocin and vasopressin were eluted in different fractions from a column of Sepharose-neurophysin I by shallow pH gradients in either direction from pH 5.8, whereas the hormones emerged in the same fractions from a Sepharose-neurophysin II column.

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