A rapid and simple screening method for methamphetamine in urine by radioimmunoassay using a 125I-labeled methamphetamine derivative.

• 稲山 誠一

• 徳永 友喜子

• 細谷 栄吉

• 中館 映夫

• 丹羽口 徹吉

• 青木 喜美子

• 斎藤 章二

抄録

N-Carboxymethylmethamphetamine, a derivative of methamphetamine, was prepared through a new synthetic pathway from ephedrine. Specific antiserum was obtained by immunization of rabbits with the conjugate of N-carboxymethylmethamphetamine with bovine serum albumin. A radioimmunoassay procedure was established using this antibody (specific for methamphetamine) and a ¹²⁵I-methamphetamine derivative. A high degree of specificity of the antibody was confirmed by testing for cross-reaction with several methamphetamine analogs, and the sensitivity was found to be 1 ng/tube. The present micro method using radioimmunoassay is highly sensitive, rapid, simple and may be useful as a micro-scale primary screening test for methamphetamine excreted in human urine, for forensic and medical purposes.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 28 (9), 2779-2782, 1980

  公益社団法人 日本薬学会

キーワード

• sensitivity

詳細情報

• CRID: 1390001204161575296
• NII論文ID: 110003624097
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.28.2779
• ISSN: 13475223
• ISSN: 00092363
• PubMed: 7460103
• Web Site: http://www.jstage.jst.go.jp/article/cpb1958/28/9/28_9_2779/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1981178207
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可