Purification and characterization of dog urinary kallikrein.

• 松田 兆史

• 宮崎 匡輔

• 藤本 幸男

• 法島 美男

• 守屋 寛

抄録

Two forms of N-α-tosyl-L-arginine methyl ester (TAME) hydrolyzing activities were found in dog urine and one of them was confirmed to be dog urinary kallikrein (DUK, E.C. 3. 4. 21. 8). Mainly by chromatographic techniques, the kallikrein was purified about 1390-fold from the dialyzed dog urine with the overall yield of 36% of vasodilator activity. The specific activities of the finally purified preparation of DUK were 1250 kallikrein units (KU)/A₂₈₀ of vasodilator activity and 11.3μmol/min/A₂₈₀ of TAME esterolytic activity, and the preparation was homogeneous on disc gel electrophoresis and ultracentrifugal analysis. The molecular weight of DUK was estimated to be approximately 2.6 to 2.9×10⁴ by vertical plate polyacrylamide gel electrophoresis, gel filtration on a Sephadex G-100 column and ultracentrifugation, and the isoelectric point of this enzyme was also determined as pI 4.2. Various properties such as pH stability, optimum pH for esterolytic activity and heat stability were similar to those of dog renal kallikrein.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 30 (7), 2512-2520, 1982

  公益社団法人 日本薬学会

キーワード

• estimation of molecular weight

詳細情報

• CRID: 1390282679138587648
• NII論文ID: 110003634632
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.30.2512
• ISSN: 13475223
• ISSN: 00092363
• PubMed: 6923779
• Web Site: http://www.jstage.jst.go.jp/article/cpb1958/30/7/30_7_2512/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1982209012
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可