Rapid effect of lymphokines on macrophage activities determined by measuring chemiluminescence.

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The effect of lymphokines on macrophage activities was studied by measuring the phorbol myristate acetate-induced luminol-dependent chemiluminescence. When thioglycolate-elicited BALB/c mouse peritoneal macrophages were incubated with lymphokines for a short time (4h), their ability to generate chemiluminescence significantly increased, while more prolonged treatment (24h) was less effective. These results show that the measurement of macrophage chemiluminescence is useful for rapid determination of lymphokines.

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