Mutarotation of D-glucose in body fluids and perfused rat liver.

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  • OKUDA JUN
    Department of Clinical Biochemistry, Faculty of Pharmacy, Meijo University
  • TAGUCHI TADAO
    Department of Clinical Biochemistry, Faculty of Pharmacy, Meijo University
  • TOMIMURA AIKO
    Clinical Laboratory, Holy Spirit Hospital

抄録

Mutarotation of n-glucose α- and β-anomers in some human body fluids, physiological salt solutions, and perfused rat liver was measured by the β-D-glucose oxidase-mutarotase method or by polarimetry. The mutarotational half-lives of α-n-glucose at 20 °C (37 °C) were as follows : whole blood, 9.0 min (2.3 min); serum, 10.6 min (2.5 min); saliva, 22.4 min (4.5 min); urine, 17.9 min (4.3 min); and distilled water, 41.0 min (9.8 min). It seems that the rapid mutarotation of D-glucose in blood may be due to the presence of mutarotase, phosphate ions, and bicarbonate ions. The mutarotation of α-n-glucose infused into isolated rat liver, warmed at 37 °C, was rapid (t1/2 = 7.2 mm), probably due to the presence of mutarotase in the liver, compared with that in the control system without liver (t1/2 = 50.3 min). The anomeric composition of D-glucose released from the perfused rat liver was almost at equilibrium.

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