萎縮病クワから師管液の採取並びにＭＬＯの検出

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  • 川北 弘
    蚕糸・昆虫農業技術研究所
  • 且原 真木
    蚕糸・昆虫農業技術研究所 岡山大学資源生物科学研究所

書誌事項

タイトル別名
  • Detection of mycoplasmalike organisms in the phloem sap collected from mulberry trees with dwarf disease.
  • イシュクビョウ クワ カラ シカンエキ ノ サイシュ ナラビニ MLO ノ ケ

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抄録

Mycoplasmalike organisms (MLOs), the pathogens of mulberry dwarf disease, were succesufully isolated by stylectomy method (cutting of insect stylets by YAG laser) using a homopterous insect, Ricania japonica. The MLOs were detectable in the phloem sap collected from diseased mulberry plants as fluorescent particles stained with 4, 6-diamidino-2-phenylindole (DAPI) under a fluorescence microscope but were not detected in the sap of healthy mulberry plants. The presence of MLOs in the phloem sap was also confirmed by electronmicroscopy.<br>On the other hand, two kinds of leafhoppers, vectors of mulberry dwarf MLOs, and other some tested hemipterous insects could not used for this laser stylectomy. Scanning-electronmicroscopical observation showed that the stylets of these leafhoppers which were not suitable for the stylectomy method were pushed out or broken after laser cutting, whwereas stylets of Ricania japonica remained firmly in the tissues.

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