Fundamental Study of Kitchen Refuse Utilization for Ethanol Fermentation by <I>Saccharomyces cerevisiae</I>

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  • PRANEETRATTANANON Suthasinee
    <I>Technology Headquarters Engineering R&D Institute, Nippon Steel Engineering CO., LTD</I>
  • KIUCHI Takafumi
    <I>Technology Headquarters Engineering R&D Institute, Nippon Steel Engineering CO., LTD</I>
  • MA Ke Dong
    <I>Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology</I>
  • WAKISAKA Minato
    <I>Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology</I>
  • MORIMURA Shigeru
    <I>Graduate School of Science and Technology, Kumamoto University</I>
  • KIDA Kenji
    <I>Graduate School of Science and Technology, Kumamoto University</I>
  • SHIRAI Yoshihito
    <I>Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology</I>

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Other Title
  • <I>Saccharomyces cerevisiae</I>による食品ゴミからのエタノール発酵に関する基礎的検討
  • Fundamental study of kichen refuse utilization for ethanol fermentation by Sacchromyces cerevisiae

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Abstract

The possibility of generating renewable bio-fuel energy through ethanol fermentation of kitchen refuse by Saccharomyces cerevisiae ATCC 26602 was established in this work. The acid-tolerant yeast S. cerevisiae ATCC 26602 was selected from among four S. cerevisiae strains as the best ethanol fermentation agent for kitchen refuse medium. The optimal kitchen refuse medium for ethanol production had a glucose concentration of only 12% (w/v) and consisted of saccharified kitchen refuse. Additional nitrogen supplementation was not necessary. A maximal ethanol concentration of 59.38 g/L with a 0.50 ethanol yield (YP/S) was obtained with the optimal medium composition in flask culture under shaking and without pH control. No significant difference was observed in ethanol production and glucose consumption between sterilized, pasteurized and non-sterilized kitchen refuse medium samples. A final ethanol concentration of 50.22 g/L, corresponding to a 0.42 ethanol yield (YP/S), was obtained using a 20-L bioreactor with a working volume of 12 L under non-sterilized conditions at an agitation rate of 100 rpm at 30°C for 18 h. Therefore, the ethanol fermentation of kitchen refuse under non-sterilized conditions by S. cerevisiae ATCC 26602 may offer an economical alternative for bio-fuel production in industrial applications.

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