局所麻酔薬の赤血球細胞膜に対する抗低張溶血作用と麻酔作用に関する研究

• 入江 修充

  日本歯科大学歯学部歯科麻酔学講座

• 砂田 勝久

  日本歯科大学歯学部歯科麻酔学講座

• 古屋 英毅

  日本歯科大学歯学部歯科麻酔学講座

書誌事項

タイトル別名

• Study on antihemolitic effect and anesthetic effect of local anesthetics on erythrocyte membrane

抄録

In the 1960s, Seeman et al, reported the effects of local anesthetics on the cell membrane, stating that low concentrations of local anesthetics strengthen the erythrocyte membrane and exert an antihemolytic effect as a result, but high concentrations of them cause hemolysis. There have been other reports concerning the interaction between anesthetic potency and hemolysis. In this study, we determined the antihemolytic effect of local anesthetics in human erythrocyte membrane and their correlation to anesthetic potency. Each local anesthetic was added at various concentrations (0-10mM) to isotonic TRIS. LA isotonic TRIS was added to 75μL of erythrocyte solution. Subsequently, hypotonic TRIS which had the same concentration of local anesthetic as the LA isotonic TRIS, was added. The resultant solution was centrifuged at 12, 000g for 5 min. The absorbance of the supernatant to a wavelength of 540nm was measured using a spectrophotometer. The antihemolytic effect of the local anesthetic at each concentration was obtained by the following formula: (Absorbance of local anesthetic at concentration of 0 mM absorbance of local anesthetic at each concentration [0.1-10mM] ) / (absorbance of a local anesthetic at concentration of 0 mM) .<BR>Procaine did not show at express antihemolysis effect. Lrdocaine indicated an antihemolysis ef-fect of 0.4mM and between 0.8 and 10mM between the control (p<0.05) . Prilocaine indicated an antihemolysis effect of 2 to 10mM between the control (p<0.05) . Mepivacaine indicated antihe-molysis effect of 2 to 4 mM between control (p<0.05) . Articaine indicated an antihemolysis effect of 0.6mM to 10mM between the control (p<0.05) . Based on these results, if an anesthetic has a direct action on nerve fiber, anesthetic potency is in the following order: lidocaine≥articaine>mepivacaine=Prilocaine.

収録刊行物

• 歯科薬物療法

  歯科薬物療法 24 (3), 95-102, 2005

  日本歯科薬物療法学会

キーワード

• 抗溶血作用
• 赤血球細胞膜
• 麻酔効力
• 局所麻酔薬
• アーティカイン

詳細情報

• CRID: 1390282679711701120
• NII論文ID: 130003840835
• DOI: 10.11263/jsotp1982.24.95
• ISSN: 18844928
• ISSN: 02881012
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可